Skip Navigation



Cerebral Cortex Advance Access published online on June 18, 2007
Cerebral Cortex, doi:10.1093/cercor/bhm092
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
18/3/553    most recent
bhm092v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Appolloni, I.
Right arrow Articles by Malatesta, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Appolloni, I.
Right arrow Articles by Malatesta, P.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2007. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Six3 Controls the Neural Progenitor Status in the Murine CNS

Irene Appolloni1,2,3, Filippo Calzolari1,2,3, Giorgio Corte1,3, Roberto Perris2,4 and Paolo Malatesta1,3

1 Istituto Nazionale per la Ricerca sul Cancro (IST), Largo Rosanna Benzi 10, 16132 Genoa, Italy, 2 Laboratory of Tumour Biology and Stem Cells, Department of Genetics, Microbiology and Anthropology, University of Parma, Parco Area delle Scienze 11/A, 43100 Parma, Italy, 3 Dipartimento di Biologia, Oncologia e Genetica, Università di Genova, Largo Rosanna Benzi 10, 16132 Genoa, Italy, 4 Laboratory for Stem Cell Research and Cellular Therapy, Division for Experimental Oncology 2, The National Cancer Institute, Aviano, CRO Via Pedemontana Occidentale 12, Aviano 33081, Italy

Address correspondence to email: paolo.malatesta{at}istge.it.

Six3, a homeodomain-containing transcriptional regulator belonging to the Six/so family, shows a defined spatiotemporal expression pattern in the developing murine telencephalon, suggesting that it may control the development of specific subsets of neural progenitors. We find that retrovirus-mediated misexpression of Six3 causes clonal expansion of isolated cortical progenitor cells by shortening their cell cycle and by prolonging their amplification period, while maintaining them in an immature precursor state. Our results show that the observed effects exerted by Six3 overexpression in mammalian brain depend strictly on the integrity of its DNA-binding domain, suggesting that Six3 action likely relies exclusively on its transcriptional activity. In vivo upregulation of Six3 expression in single progenitor cells of the embryonic telencephalon keeps them in an undifferentiated state. Our observations point to a role of Six3 in the control of the subtle equilibrium between proliferation and differentiation of defined precursor populations during mammalian neurogenesis.

Key Words: homeobox transcription factors • neural stem cells • retroviral lineage tracing


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
M. Buscarlet, A. Perin, A. Laing, J. M. Brickman, and S. Stifani
Inhibition of Cortical Neuron Differentiation by Groucho/TLE1 Requires Interaction with WRPW, but Not Eh1, Repressor Peptides
J. Biol. Chem., September 5, 2008; 283(36): 24881 - 24888.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.