Skip Navigation



Cerebral Cortex Advance Access published online on June 24, 2004
Cerebral Cortex, doi:10.1093/cercor/bhh101
© 2004 by Oxford University Press
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
14/12/1398    most recent
bhh101v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Muly, E. C.
Right arrow Articles by Greengard, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Muly, E. C.
Right arrow Articles by Greengard, P.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Article

Subcellular Distribution of Neurabin Immunolabeling in Primate Prefrontal Cortex: Comparison with Spinophilin

E. Chris Muly 1*, Patrick Allen 2, Maney Mazloom 3, Zina Aranbayeva 3, Audrey T. Greenfield 4, Paul Greengard 4

1 Department of Psychiatry and Behavioral Sciences, Emory University, Atlanta, GA 30329, USA; Division of Neuroscience, Yerkes National Primate Research Center, Atlanta, GA 30329, USA
2 Department of Psychiatry, Yale University School of Medicine, New Haven, CT 06508, USA
3 Division of Neuroscience, Yerkes National Primate Research Center, Atlanta, GA 30329, USA
4 Laboratory of Molecular and Cellular Neuroscience, The Rockefeller University, New York, NY 10021, USA

* To whom correspondence should be addressed. E-mail: ecmuly{at}rmy.emory.edu.


  Abstract

Prefrontal cortical functioning depends on dopaminergic neurotransmission, which in turn depends on a complex signal transduction pathway including protein phosphatase-1 (PP1). Targeted localization of PP1 by the scaffolding proteins, spinophilin and neurabin, is critical for dopaminergic modulation of glutamate neurotransmission. In this study, we report the preparation of an antiserum to neurabin, use it to study the subcellular localization of neurabin and compare that to our previous study of spinophilin, a closely related PP1 scaffold. Neurabin is found predominately in dendritic spines, but is also found in other compartments, including dendrites, axons, terminals and glia. This distribution contrasts with that of spinophilin in that neurabin is found in axon terminals where spinophilin is absent, and in parvalbumin-containing interneuron dendrites there is no significant neurabin though these dendrites contain substantial spinophilin. Within the dendritic spine compartment, however, the two proteins are similarly distributed. Both neurabin and spinophilin are concentrated in spines, and double-labeling reveals that they co-localize in most spines. Furthermore, post-embedding immunogold labeling demonstrates that within a spine, neurabin is distributed in the same pattern as spinophilin, concentrated in the postsynaptic density and the 100 nm just below. These results indicate that neurabin and spinophilin share important similarities and differences in their patterns of distribution. Varying patterns of scaffold localization may play an important role in determining the content and action of signal transduction pathways in different neuronal populations or compartments.

Keywords: interneuron; post-embedding immunogold; postsynaptic density; protein phosphatase-1; scaffolding protein; spinophilin.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 FASEB J.Home page
L. C. Carmody, A. J. Baucum II, M. A. Bass, and R. J. Colbran
Selective targeting of the {gamma}1 isoform of protein phosphatase 1 to F-actin in intact cells requires multiple domains in spinophilin and neurabin
FASEB J, June 1, 2008; 22(6): 1660 - 1671.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
J. Prange-Kiel, H. Jarry, M. Schoen, P. Kohlmann, C. Lohse, L. Zhou, and G. M. Rune
Gonadotropin-releasing hormone regulates spine density via its regulatory role in hippocampal estrogen synthesis
J. Cell Biol., January 28, 2008; 180(2): 417 - 426.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M.-H. Liang and D.-M. Chuang
Regulation and Function of Glycogen Synthase Kinase-3 Isoforms in Neuronal Survival
J. Biol. Chem., February 9, 2007; 282(6): 3904 - 3917.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
W. Hung, C. Hwang, M. D. Po, and M. Zhen
Neuronal polarity is regulated by a direct interaction between a scaffolding protein, Neurabin, and a presynaptic SAD-1 kinase in Caenorhabditis elegans
Development, January 15, 2007; 134(2): 237 - 249.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
D. Orioli, I. N. Colaluca, M. Stefanini, S. Riva, C. G. Dotti, and F. A. Peverali
Rac3-induced Neuritogenesis Requires Binding to Neurabin I
Mol. Biol. Cell, May 1, 2006; 17(5): 2391 - 2400.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
R. T. Terry-Lorenzo, D. W. Roadcap, T. Otsuka, T. A. Blanpied, P. L. Zamorano, C. C. Garner, S. Shenolikar, and M. D. Ehlers
Neurabin/Protein Phosphatase-1 Complex Regulates Dendritic Spine Morphogenesis and Maturation
Mol. Biol. Cell, May 1, 2005; 16(5): 2349 - 2362.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.