Cerebral Cortex Advance Access originally published online on May 27, 2004
Cerebral Cortex 2004 14(11):1276-1286; doi:10.1093/cercor/bhh089
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© Oxford University Press 2004
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Selective Neurofilament (SMI-32, FNP-7 and N200) Expression in Subpopulations of Layer V Pyramidal Neurons In Vivo and In Vitro
1 Department of Human Anatomy and Genetics, University of Oxford, South Park Road, Oxford, OX1 3QX, UK, 2 Swiss Institute for Experimental Cancer Research, Ch. Boveresses 155, 1066 Epalinges, Switzerland, 3 Brain Research Institute, University of Zurich, Winterthurerstrasse 190, CH-8057, Zurich, Switzerland, 4 Institut de Biologie Cellulaire et de Morphologie, Rue Du Bugnon 9, 1005 Lausanne, Switzerland
There are two main types of layer V pyramidal neurons in rat cortex. Type I neurons have tufted apical dendrites extending into layer I, produce bursts of action potentials and project to subcortical targets (spinal cord, superior colliculus and pontine nuclei). Type II neurons have apical dendrites, which arborize in layers II–IV, do not produce bursts of action potentials and project to ipsilateral and contralateral cortex. The specific expression of different genes and proteins in these two distinct layer V neurons is unknown. To distinguish between distinct subpopulations, fluorescent microspheres were injected into subcortical targets (labeling type I neurons) or primary somatosensory cortex (labeling type II neurons) of adult rats. After transport, cortical sections were processed for immunohistochemistry using various antibodies. This study demonstrated that antigens recognized by SMI-32, N200 and FNP-7 antibodies were only expressed in subcortical (type I) — but not in contralateral (type II) — projecting neurons. NR1, NR2a/b, PLCß1, BDNF, NGF and TrkB antigens were highly expressed in all neuronal subpopulations examined. Organotypic culture experiments demonstrated that the development of neurofilament expression and laminar specificity does not depend on the presence of the subcortical targets. This study suggests specific markers for the subcortical projecting layer V neuron subpopulations.
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