Cerebral Cortex, Vol. 11, No. 7, 666-678,
July 2001
© 2001 Oxford University Press
Vertical Bias in Dendritic Trees of Non-pyramidal Neocortical Neurons Expressing GAD67–GFP In Vitro
1 Department of Neurobiology and Anatomy, , 2 Departments of Otolaryngology and Biochemistry, , 3 Sensory Neuroscience Research Center and the , 4 Neuroscience Graduate Program, West Virginia University, Morgantown, WV 26506, USA and , 5 Laboratory of Molecular Biology and Genetics, Institute of Experimental Medicine, Hungarian Academy of Sciences, H-1450 Budapest, Hungary
The neocortical neuropil has a strong vertical (orthogonal to pia) orientation, constraining the intracortical flow of information and forming the basis for the functional parcellation of the cortex into semi-independent vertical columns or ‘modules’. Apical dendrites of excitatory pyramidal neurons are a major component of this vertical neuropil, but the extent to which inhibitory, GABAergic neurons conform to this structural and functional design is less well documented. We used a gene gun to transfect organotypic slice cultures of mouse and rat neocortex with the enhanced green fluorescent protein (eGFP) gene driven by the promoter for glutamic acid decarboxylase 67 (GAD67), an enzyme expressed exclusively in GABAergic cells. Many GAD67–GFP expressing cells were highly fluorescent, and their dendritic morphologies and axonal patterns, revealed in minute detail, were characteristic of GABAergic neurons. We traced 150 GFP-expressing neurons from confocal image stacks, and estimated the degree of vertical bias in their dendritic trees using a novel computational metric. Over 70% of the neurons in our sample had dendritic trees with a highly significant vertical bias. We conclude that GABAergic neurons make an important contribution to the vertical neocortical neuropil, and are likely to integrate synaptic inputs from axons terminating within their own module.
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