Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (8)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Hill, E.
Right arrow Articles by Tan, S.-S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hill, E.
Right arrow Articles by Tan, S.-S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Cerebral Cortex, Vol. 10, No. 11, 1132-1142, November 2000
© 2000 Oxford University Press

Glutamate, GABA and Precursor Amino Acids in Adult Mouse Neocortex: Cellular Diversity Revealed by Quantitative Immunocytochemistry

Elisa Hill, Michael Kalloniatis1 and Seong-Seng Tan

The Howard Florey Institute, University of Melbourne, 1 The Department of Optometry and Vision Sciences, The University of Melbourne, Parkville, 3010, Victoria, Australia

Glutamate is an important amino acid in the neocortex for metabolic and neurotransmitter functions. The objective of this study was to detect variations in cellular glutamate content using quantitative immunocytochemistry. We show that glutamate is present in almost all cortical cells and coexists with other amino acids such as aspartate, glutamine or {gamma}-aminobutyric acid (GABA). The patterns of aspartate and glutamine content suggests that there are no purely aspartatergic or glutaminergic neurons. GABAergic neurons showed variable levels of the precursors such as glutamate, glutamine and aspartate. Comparison of immunoreactive patterns between two cortical areas did not detect any statistically significant differences. The mean cellular intensity for GABA and glutamate was constant across different layers. Surprisingly, we found that GABAergic neurons could coexist with either low or high levels of glutamate, suggesting that metabolic levels of glutamate in these neurons could be variable. Alternatively, some GABA neurons may utilize both GABA and glutamate for neurotransmission. We show that when variations in amino acid content are separately mapped onto individual cells, co-registration is a useful technique for reporting heterogeneity among cortical cells.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Cereb CortexHome page
C.-H. Chan, L. N. Godinho, D. Thomaidou, S.-S. Tan, M. Gulisano, and J. G. Parnavelas
Emx1 is a Marker for Pyramidal Neurons of the Cerebral Cortex
Cereb Cortex, December 1, 2001; 11(12): 1191 - 1198.
[Abstract] [Full Text] [PDF]

Home page
 Cereb CortexHome page
E. Hill, M. Kalloniatis, and S.-S. Tan
Cellular Diversity in Mouse Neocortex Revealed by Multispectral Analysis of Amino Acid Immunoreactivity
Cereb Cortex, August 1, 2001; 11(8): 679 - 690.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.