Cerebral Cortex 1991; 1:48-61
© Oxford University Press 1991
research-article |
Patch-Clamp Studies of Voltage-Gated Currents in Identified Neurons of the Rat Cerebral Cortex
Department of Neurology, Stanford University School of Medicine Stanford, California
Address correspondence and reprint requests to Dr. Owen P. Hamill, Section of Neurobiology and Behavior, Seeley G. Mudd Hall, Cornell University, Ithaca, NY 14853.
In the cerebral cortex, neurons can be classified into 2 broad morphological classes, referred to as pyramidal and nonpyramidal (stellate) cells, which correspond to functional classes of projection neurons and local circuit interneurons, respectively. In this study, we demonstrate that specific morphological, immunohistochemical, and physiological features, that allow class dis tinction of neurons in situ, are retained in acutely isolated neocortical neurons. Furthermore, voltage-clamp analysis with patch-clamp techniques indicate the differences in functional properties in adult neurons, reflect cell-specific, developmental changes in the density and type of specific classes of Na+ K+ and Ca2+ channels expressed. The differences in channel properties contribute to the different input-output relations of neocortical neurons, which enable inhibitory neurons to follow excitatory inputs faithfully and projection neurons to have more integrative roles.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  C. Mollinari, L. Ricci-Vitiani, M. Pieri, C. Lucantoni, A. M. Rinaldi, M. Racaniello, R. De Maria, C. Zona, R. Pallini, D. Merlo, et al. Downregulation of thymosin {beta}4 in neural progenitor grafts promotes spinal cord regeneration J. Cell Sci., November 15, 2009; 122(22): 4195 - 4207. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. K. Esser, S. Hill, and G. Tononi Breakdown of Effective Connectivity During Slow Wave Sleep: Investigating the Mechanism Underlying a Cortical Gate Using Large-Scale Modeling J Neurophysiol, October 1, 2009; 102(4): 2096 - 2111. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Bar-Yehuda and A. Korngreen Space-Clamp Problems When Voltage Clamping Neurons Expressing Voltage-Gated Conductances J Neurophysiol, March 1, 2008; 99(3): 1127 - 1136. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Gibson, A. F. Bartley, and K. M. Huber Role for the Subthreshold Currents ILeak and IH in the Homeostatic Control of Excitability in Neocortical Somatostatin-Positive Inhibitory Neurons J Neurophysiol, July 1, 2006; 96(1): 420 - 432. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N.-L. R. Han, J.-S. Ye, A. C. H. Yu, and F.-S. Sheu Differential Mechanisms Underlying the Modulation of Delayed-Rectifier K+ Channel in Mouse Neocortical Neurons by Nitric Oxide J Neurophysiol, April 1, 2006; 95(4): 2167 - 2178. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G.-L. Wang, G.-X. Wang, S. Yamamoto, L. Ye, H. Baxter, J. R Hume, and D. Duan Molecular mechanisms of regulation of fast-inactivating voltage-dependent transient outward K+ current in mouse heart by cell volume changes J. Physiol., October 15, 2005; 568(2): 423 - 443. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Azouz Dynamic Spatiotemporal Synaptic Integration in Cortical Neurons: Neuronal Gain, Revisited J Neurophysiol, October 1, 2005; 94(4): 2785 - 2796. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Rudolph, J. G. Pelletier, D. Pare, and A. Destexhe Characterization of Synaptic Conductances and Integrative Properties During Electrically Induced EEG-Activated States in Neocortical Neurons In Vivo J Neurophysiol, October 1, 2005; 94(4): 2805 - 2821. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Rudolph, Z. Piwkowska, M. Badoual, T. Bal, and A. Destexhe A Method to Estimate Synaptic Conductances From Membrane Potential Fluctuations J Neurophysiol, June 1, 2004; 91(6): 2884 - 2896. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. L. Picken Bahrey and W. J. Moody Early Development of Voltage-Gated Ion Currents and Firing Properties in Neurons of the Mouse Cerebral Cortex J Neurophysiol, April 1, 2003; 89(4): 1761 - 1773. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A Rhodes and R. R Llinas Apical tuft input efficacy in layer 5 pyramidal cells from rat visual cortex J. Physiol., October 1, 2001; 536(1): 167 - 187. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J J. Zhu Maturation of layer 5 neocortical pyramidal neurons: amplifying salient layer 1 and layer 4 inputs by Ca2+ action potentials in adult rat tuft dendrites J. Physiol., August 1, 2000; 526(3): 571 - 587. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M Bekkers Properties of voltage-gated potassium currents in nucleated patches from large layer 5 cortical pyramidal neurons of the rat J. Physiol., June 15, 2000; 525(3): 593 - 609. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Korngreen and B. Sakmann Voltage-gated K+ channels in layer 5 neocortical pyramidal neurones from young rats: subtypes and gradients J. Physiol., June 15, 2000; 525(3): 621 - 639. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. C. Bush, D. A. Prince, and K. D. Miller Increased Pyramidal Excitability and NMDA Conductance Can Explain Posttraumatic Epileptogenesis Without Disinhibition: A Model J Neurophysiol, October 1, 1999; 82(4): 1748 - 1758. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M. Talley, L. L. Cribbs, J.-H. Lee, A. Daud, E. Perez-Reyes, and D. A. Bayliss Differential Distribution of Three Members of a Gene Family Encoding Low Voltage-Activated (T-Type) Calcium Channels J. Neurosci., March 15, 1999; 19(6): 1895 - 1911. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. Locke and J. M. Nerbonne Three Kinetically Distinct Ca2+-Independent Depolarization-Activated K+ Currents in Callosal-Projecting Rat Visual Cortical Neurons J Neurophysiol, November 1, 1997; 78(5): 2309 - 2320. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. Locke and J. M. Nerbonne Role of Voltage-Gated K+ Currents in Mediating the Regular-Spiking Phenotype of Callosal-Projecting Rat Visual Cortical Neurons J Neurophysiol, November 1, 1997; 78(5): 2321 - 2335. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Baro, R. M. Levini, M. T. Kim, A. R. Willms, C. C. Lanning, H. E. Rodriguez, and R. M. Harris-Warrick Quantitative Single-Cell-Reverse Transcription-PCR Demonstrates That A-Current Magnitude Varies as a Linear Function of shal Gene Expression in Identified Stomatogastric Neurons J. Neurosci., September 1, 1997; 17(17): 6597 - 6610. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Massengill, M. A. Smith, D. I. Son, and D. K. O'Dowd Differential Expression of K4-AP Currents and Kv3.1 Potassium Channel Transcripts in Cortical Neurons that Develop Distinct Firing Phenotypes J. Neurosci., May 1, 1997; 17(9): 3136 - 3147. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-W. Jeong and R. D. Wurster Calcium Channel Currents in Acutely Dissociated Intracardiac Neurons From Adult Rats J Neurophysiol, April 1, 1997; 77(4): 1769 - 1778. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Nick, L. K. Kaczmarek, and T. J. Carew Ionic Currents Underlying Developmental Regulation of Repetitive Firing in Aplysia Bag Cell Neurons J. Neurosci., December 1, 1996; 16(23): 7583 - 7598. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Castro-Alamancos and B. W. Connors Cellular Mechanisms of the Augmenting Response: Short-Term Plasticity in a Thalamocortical Pathway J. Neurosci., December 1, 1996; 16(23): 7742 - 7756. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. I. Banks, L. B. Haberly, and M. B. Jackson Layer-Specific Properties of the Transient K Current (IA) in Piriform Cortex J. Neurosci., June 15, 1996; 16(12): 3862 - 3876. [Abstract] [Full Text] [PDF]
|
|