Cerebral Cortex, Vol 7, 546-558, Copyright © 1997 by Oxford University Press
R Yuste, DW Tank and D Kleinfeld
The computations performed within cortex are likely to be determined by its
internal dynamics in addition to its pattern of afferent input. As a step
toward characterizing these dynamics, we have imaged electrical activity in
slices from rat primary visual cortex stained with the voltage-sensitive
dye di-4-ANEPPS. In response to electrical stimulation two fluorescence
signals of similar maximum amplitude are elicited, (i) A fast signal that
peaks in a few milliseconds, is dependent on membrane voltage, and has a
significant presynaptic component. This signal can be used to image
electrical activity ratiometrically. (ii) A slow signal that peaks a few
seconds after stimulation, does not reflect voltage changes, and may
originate from changes in scattering properties of the slice and from
interactions of the dye with the cells. The spatial pattern of fast signals
obtained in response to focal stimulation of coronal slices is consistent
with known interlaminar projection patterns. In tangential slices, imaging
of fast signals reveals clustered horizontal responses. Finally, imaging of
fast signals during epileptiform activation of the disinhibited circuit
reveals propagating responses, without evidence for modular activation.
ARTICLES
Functional study of the rat cortical microcircuitry with voltage- sensitive dye imaging of neocortical slices
Department of Biological Sciences, Columbia University, New York, NY 10027, USA.
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