Cerebral Cortex, Vol 7, 293-302, Copyright © 1997 by Oxford University Press
JF Cavanagh, MC Mione, IS Pappas and JG Parnavelas
Basic fibroblast growth factor (bFGF) has been shown to influence the
survival, proliferation and differentiation of a variety of cell types in
the nervous system. In this investigation we have examined the action of
bFGF on: (i) the rate of proliferation; (ii) cell cycle parameters; (iii)
the maintenance of cell division; (iv) the recruitment of quiescent cells;
and (v) the degree of differentiation of cortical progenitor cells in
cultures prepared from E16 rat embryos. The proliferation rate (labelling
index) of cortical progenitor cells doubled in the presence of bFGF over 48
h. However, the lengths of the cell cycle phases were unchanged. Clones
marked with a recombinant retrovirus on the first day in vitro (DIV) grew
significantly larger in the presence of bFGF. Furthermore, many of the
clones examined in control cultures had ceased to divide after a maximum of
four cell cycles, whereas almost all clonally related cells were still
dividing in the presence of bFGF 4 days later, i.e. for at least six cell
cycles. Basic FGF also stimulated the division of quiescent progenitor
cells, which otherwise would have differentiated or undergone cell death.
The degree of neuronal and glial differentiation was studied after 5 DIV
using MAP-2 and GFAP immunocytochemistry. In the presence of bFGF, the
percentage of MAP-2-labelled cells was less than half that of control
cultures, whereas the number of cells immunoreactive for nestin (a marker
of progenitor cells) remained very high. Cells immunoreactive for GFAP were
present in bFGF-treated cultures, yet were extremely rare in control
conditions. These experiments show that bFGF, a potent mitogen for cortical
progenitor cells, has no effects on the parameters of their cell cycle but
extends their proliferative capability, promotes their survival and delays
their differentiation into neurons.
ARTICLES
Basic fibroblast growth factor prolongs the proliferation of rat cortical progenitor cells in vitro without altering their cell cycle parameters
Department of Anatomy and Developmental Biology, University College London, UK.
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