Cerebral Cortex, Vol 7, 166-177, Copyright © 1997 by Oxford University Press
JC Horton and DR Hocking
A pattern of alternating light and dark columns was observed in wet,
unstained sections of macaque striate cortex after monocular enucleation.
The columns were clearest in layer IV, but could be detected through the
full thickness of the cortex. Subsequent processing for cytochrome oxidase
(CO) showed that the light columns in wet sections viewed under darkfield
illumination matched the ocular dominance columns serving the enucleated
eye. These columns labeled preferentially with an antibody to myelin basic
protein, suggesting that greater myelin content accounted for their
brighter appearance. However, when sections were counterstained with luxol
fast blue, Gallyas and Woelcke myelin techniques, the enucleated eye's
columns appeared pale. It is unclear why classical myelin stains and myelin
basic protein immunohistochemistry yielded opposite results. Discrepant
patterns of myelin distribution were also found in normal animals using
different myelin stains. Luxol fast blue showed homogeneous staining in
layer IVc of macaque striate cortex, but the Gallyas stain revealed a
pattern of thin pale bands alternating with wide dark bands, matching the
pattern seen with the Liesegang stain. The CO patches in layers II and III
fit in register with the wide dark myelin bands. In layers II and III of
striate cortex, the Gallyas and luxol fast blue methods both labeled the CO
patches. However, in squirrel monkey V2 the Gallyas stain labeled the pale
CO stripes, whereas luxol fast blue labeled the dark CO stripes. These
results indicate that pattern of myelin staining in visual cortex can vary
according to the choice of technique, and may not reflect the true
distribution of myelin. Studies of myeloarchitecture should employ a
variety of myelin techniques, including examination of unstained sections,
to obtain the most accurate picture of cortical myelin content.
ARTICLES
Myelin patterns in V1 and V2 of normal and monocularly enucleated monkeys
Beckman Vision Center, University of California - San Francisco 94143- 0730, USA.
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