Cerebral Cortex Advance Access originally published online on November 13, 2008
Cerebral Cortex 2009 19(8):1738-1750; doi:10.1093/cercor/bhn195
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Novel Markers Reveal Subpopulations of Subplate Neurons in the Murine Cerebral Cortex
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1 Department of Physiology, Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, 2 MRC Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, 3 Department of Microbiology, Immunology and Genetics, Developmental Neurobiology Unit, Université Catholique de Louvain, B-1348 Brussels, Belgium, 4 Department of Anatomy and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK, 5 Department of Molecular Neurobiology and Center for the Molecular Physiology of the Brain, Max Planck Institute of Experimental Medicine, Hermann-Rein Strasse 3, D-37075 Göttingen, Germany, 6 Department of Cellular and Molecular Neuroscience, Imperial College School of Medicine, Hammersmith Campus, Du Cane Road, London SW7 2AZ, UK
Address correspondence to email: zoltan.molnar{at}anat.ox.ac.uk.
The subplate lays the foundation of the developing cerebral cortex, and abnormalities have been suggested to contribute to various brain developmental disorders. The causal relationship between cellular pathologies and cognitive disorders remains unclear, and therefore, a better understanding of the role of subplate cells in cortical development is essential. Only by determining the molecular taxonomy of this diverse class of neurons can we identify the subpopulations that may contribute differentially to cortical development. We identified novel markers for murine subplate cells by comparing gene expression of subplate and layer 6 of primary visual and somatosensory cortical areas of postnatal day (P)8 old mice using a microarray-based approach. We examined the utility of these markers in well-characterized mutants (reeler, scrambler, and p35-KO) where the subplate is displaced in relation to the cortical plate. In situ hybridization or immunohistochemistry confirmed subplate-selective expression of complexin 3, connective tissue growth factor, nuclear receptor–related 1/Nr4a2, and monooxygenase Dbh-like 1 while transmembrane protein 163 also had additional expression in layer 5, and DOPA decarboxylase was also present in the white matter. Localization of marker-positive cells in the reeler and p35-KO cortices suggests different subpopulations of subplate cells. These new markers open up possibilities for further identification of subplate subpopulations in research and in neuropathological diagnosis.
Key Words: CTGF • DDC • Nurr1 • p35-KO • reeler mutant • subplate
Anna Hoerder-Suabedissen and Wei Zhi Wang are joint first authors, contributed equally to this work
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