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Cerebral Cortex, Vol. 12, No. 5, 552-564, May 2002
© 2002 Oxford University Press

Northern Blot and In Situ Hybridization Analyses of MARCKS mRNA Expression in the Cerebral Cortex of the Macaque Monkey

Noriyuki Higo1,2, Takao Oishi1, Akiko Yamashita3, Keiji Matsuda1 and Motoharu Hayashi4

1 Neuroscience Research Institute, National Institute of Advanced Industrial Science and Technology, Umezono, Tsukuba, Ibaraki 305-8568, , 2 Department of Physiology, University of Tsukuba School of Medicine, Tennodai, Tsukuba, Ibaraki 305-8575, , 3 Department of Anatomy, Nihon University School of Medicine, Oyaguchi-Kamimachi, Itabashi, Tokyo 173-8610 and , 4 Department of Cellular and Molecular Biology, Primate Research Institute, Kyoto University, Kanrin, Inuyama, Aichi 484-8506, Japan

T. Oishi, Neuroscience Research Institute, AIST, Tsukuba Central 2, Umezono 1-1-1, Tsukuba, Ibaraki 305-8568, Japan. Email: t.oishi{at}aist.go.jp.

Myristoylated alanine-rich C-kinase substrate (MARCKS) is a major substrate for protein kinase C, and is involved in synaptic plasticity. Using both Northern blot and in situ hybridization techniques, we investigated whether MARCKS expression varied according to the cerebral region, including the hippocampal formation, or according to the type of neuron. Northern blot analysis showed that the MARCKS mRNA level was higher in the association areas than in the primary sensory and motor areas of the cerebral neocortex. MARCKS mRNA levels in the hippocampus and the amygdala were as high as those in the association areas. The in situ hybridization experiments confirmed the Northern blot results and showed the distribution and characteristics of MARCKS mRNA-positive neurons. In the association areas of the neocortex, prominent signals were observed in neurons in layers II–VI. In the primary areas, prominent signals were restricted to neurons in layers IV-VI. In the hippocampus, the most intense hybridization signals were observed in neurons in the granule cell layer of the dentate gyrus. The observed region-specific expression might reflect functional specialization for plasticity in individual regions of the monkey cerebral cortex.


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