Cerebral Cortex, Vol. 12, No. 12, 1225-1236,
December 2002
© 2002 Oxford University Press
Selective Expression of Doublecortin and LIS1 in Developing Human Cortex Suggests Unique Modes of Neuronal Movement
Department of Anatomy, Faculty of Medicine, University La Laguna, La Laguna/Tenerife, Spain and , 1 Department of Neurosciences, University of California–San Diego, La Jolla, CA, USA
Address correspondence to Gundela Meyer, Department of Anatomy, Faculty of Medicine, University of La Laguna, 38071 La Laguna/Tenerife, Spain. Email: gmeyer{at}ull.es.
The genes doublecortin (DCX) and LIS1 are required for proper cortical neuronal migration and differentiation in humans. Here, we study the expression pattern of the encoded proteins of these genes in developing human brain. LIS1 stained virtually all migrating neurons throughout periods of development. Initially, DCX extensively overlapped with Reelin in early preplate stage in radially oriented columns of cells in the ventricular zone, whereas at later stages, the majority of DCX-positive cells were horizontally oriented. During the cortical plate stage, two opposite patterns of DCX expression were found: in radially oriented apical processes, presumably of pyramidal cells in the cortical plate, and in non-radially oriented mono- or bipolar neurons with migratory morphologies in the deep compartments of the cerebral wall. The extensive co-localization of DCX and Calretinin in non-radially oriented neurons suggested a non-pyramidal phenotype. These cells assumed a more vertical orientation upon entering the subplate. In addition, DCX was expressed by cells in the subpial granular layer and by Cajal–Retzius cells. In a 19 week human fetal cortex with a LIS1 mutation, the number of Reelin-expressing Cajal–Retzius cells was reduced and their morphology was abnormal. DCX was expressed by cells in all regions, but in extremely low numbers, suggesting that LIS1 deficiency adversely affects the migration and differentiation of DCX- and Reelin-positive neurons.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  N. Bayatti, J. A. Moss, L. Sun, P. Ambrose, J. F. H. Ward, S. Lindsay, and G. J. Clowry A Molecular Neuroanatomical Study of the Developing Human Neocortex from 8 to 17 Postconceptional Weeks Revealing the Early Differentiation of the Subplate and Subventricular Zone Cereb Cortex, July 1, 2008; 18(7): 1536 - 1548. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. L. Walker, T. Yasuda, D. J. Adams, and P. F. Bartlett The Doublecortin-Expressing Population in the Developing and Adult Brain Contains Multipotential Precursors in Addition to Neuronal-Lineage Cells J. Neurosci., April 4, 2007; 27(14): 3734 - 3742. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. L. Ramos, J. Bai, and J. J. LoTurco Heterotopia Formation in Rat but Not Mouse Neocortex after RNA Interference Knockdown of DCX Cereb Cortex, September 1, 2006; 16(9): 1323 - 1331. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Kappeler, Y. Saillour, J.-P. Baudoin, F. P. D. Tuy, C. Alvarez, C. Houbron, P. Gaspar, G. Hamard, J. Chelly, C. Metin, et al. Branching and nucleokinesis defects in migrating interneurons derived from doublecortin knockout mice Hum. Mol. Genet., May 1, 2006; 15(9): 1387 - 1400. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Bystron, Z. Molnar, V. Otellin, and C. Blakemore Tangential Networks of Precocious Neurons and Early Axonal Outgrowth in the Embryonic Human Forebrain J. Neurosci., March 16, 2005; 25(11): 2781 - 2792. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Friocourt, A. Koulakoff, P. Chafey, D. Boucher, F. Fauchereau, J. Chelly, and F. Francis Doublecortin Functions at the Extremities of Growing Neuronal Processes Cereb Cortex, June 1, 2003; 13(6): 620 - 626. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kato and W. B. Dobyns Lissencephaly and the molecular basis of neuronal migration Hum. Mol. Genet., April 2, 2003; 12(90001): R89 - 96. [Abstract] [Full Text] [PDF]
|
|