Cerebral Cortex, Vol. 11, No. 8, 744-753,
August 2001
© 2001 Oxford University Press
GABAB Receptors Mediate Motility Signals for Migrating Embryonic Cortical Cells
Laboratory of Neurophysiology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892 and , 1 Department of Chemistry, University of Florida, Gainesville, FL 32611, USA
During development, postmitotic neurons migrate from germinal regions into the cortical plate (cp), where lamination occurs. In rats, GABA is transiently expressed in the cp, near target destinations for migrating neurons. In vitro GABA stimulates neuronal motility, suggesting cp cells release GABA, which acts as a chemoattractant during corticogenesis. Pharmacological studies indicate GABA stimulates migration via GABAB-receptor (GABAB-R) activation. Using immunohistochemistry, RT-PCR and Western blotting, we examined embryonic cortical cell expression of GABAB-Rs in vivo. At E17, GABAB-R1+ cells were identified in the ventricular zone (vz) and cp. RT-PCR and Western blotting demonstrated the presence of GABAB-R1a and GABAB-R1b mRNA and proteins. Using immuno- cytochemistry, GABAB-R expression was examined in vz and cp cell dissociates before and after migration to GABA in an in vitro chemotaxis assay. GABA-induced migration resulted in an increase of GABAB-R+ cells in the migrated population. While <20% of each starting dissociate was GABAB-R+, >70% of migrated cells were immunopositive. We used a microchemotaxis assay to analyze cp cell release of diffusible chemotropic factor(s). In vitro, cp dissociates induced vz cell migration in a cell density-dependent manner that was blocked by micromolar saclofen (a GABAB-R antagonist). HPLC demonstrated cp cells release micromolar levels of GABA and taurine in several hours. Micromolar levels of both molecules stimulated cell migration that was blocked by micromolar saclofen. Thus, migratory cortical cells express GABAB-Rs, cp cells release GABA and taurine, and both molecules stimulate cortical cell movement. Together these findings suggest GABA and/or taurine act as chemoattractants for neurons during rat cortical histogenesis via mechanisms involving GABAB-Rs.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Iguchi, K. Sakata, K. Yoshizaki, K. Tago, N. Mizuno, and H. Itoh Orphan G Protein-coupled Receptor GPR56 Regulates Neural Progenitor Cell Migration via a G{alpha}12/13 and Rho Pathway J. Biol. Chem., May 23, 2008; 283(21): 14469 - 14478. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Cancedda, H. Fiumelli, K. Chen, and M.-m. Poo Excitatory GABA Action Is Essential for Morphological Maturation of Cortical Neurons In Vivo J. Neurosci., May 9, 2007; 27(19): 5224 - 5235. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Heck, W. Kilb, P. Reiprich, H. Kubota, T. Furukawa, A. Fukuda, and H. J. Luhmann GABA-A Receptors Regulate Neocortical Neuronal Migration In Vitro and In Vivo Cereb Cortex, January 1, 2007; 17(1): 138 - 148. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Gressens Neuronal Migration Disorders J Child Neurol, December 1, 2005; 20(12): 968 - 971. [Abstract] [PDF]
|
|
|
|
 |
|
 M. T. Ghorbel, K. G. Becker, and J. M. Henley Profile of changes in gene expression in cultured hippocampal neurones evoked by the GABAB receptor agonist baclofen Physiol Genomics, June 16, 2005; 22(1): 93 - 98. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Wonders and S. A. Anderson Cortical Interneurons and Their Origins Neuroscientist, June 1, 2005; 11(3): 199 - 205. [Abstract] [PDF]
|
|
|
|
|
|
J.-B. Manent, M. Demarque, I. Jorquera, C. Pellegrino, Y. Ben-Ari, L. Aniksztejn, and A. Represa A Noncanonical Release of GABA and Glutamate Modulates Neuronal Migration J. Neurosci., May 11, 2005; 25(19): 4755 - 4765. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Moya and M. Valdeolmillos Polarized Increase of Calcium and Nucleokinesis in Tangentially Migrating Neurons Cereb Cortex, June 1, 2004; 14(6): 610 - 618. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. G. Costa, L. Steardo, and V. Cuomo Structural Effects and Neurofunctional Sequelae of Developmental Exposure to Psychotherapeutic Drugs: Experimental and Clinical Aspects Pharmacol. Rev., March 1, 2004; 56(1): 103 - 147. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Tanaka, Y. Nakaya, Y. Yanagawa, K. Obata, and F. Murakami Multimodal tangential migration of neocortical GABAergic neurons independent of GPI-anchored proteins Development, December 1, 2003; 130(23): 5803 - 5813. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Lopez-Bendito, R. Lujan, R. Shigemoto, P. Ganter, O. Paulsen, and Z. Molnar Blockade of GABAB Receptors Alters the Tangential Migration of Cortical Neurons Cereb Cortex, September 1, 2003; 13(9): 932 - 942. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. L. Picken Bahrey and W. J. Moody Early Development of Voltage-Gated Ion Currents and Firing Properties in Neurons of the Mouse Cerebral Cortex J Neurophysiol, April 1, 2003; 89(4): 1761 - 1773. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Molnar, G. Lopez-Bendito, J. Small, L. D. Partridge, C. Blakemore, and M. C. Wilson Normal Development of Embryonic Thalamocortical Connectivity in the Absence of Evoked Synaptic Activity J. Neurosci., December 1, 2002; 22(23): 10313 - 10323. [Abstract] [Full Text] [PDF]
|
|