Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (18)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Hanganu, I. L.
Right arrow Articles by Luhmann, H. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hanganu, I. L.
Right arrow Articles by Luhmann, H. J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Cerebral Cortex, Vol. 11, No. 5, 400-410, May 2001
© 2001 Oxford University Press

Spontaneous Synaptic Activity of Subplate Neurons in Neonatal Rat Somatosensory Cortex

Ileana L. Hanganu, Werner Kilb and Heiko J. Luhmann

Institute of Neurophysiology, Heinrich-Heine-University, Düsseldorf, Germany

Subplate neurons play an important role in early cortical development. To investigate whether these transient neurons receive synaptic inputs, we performed whole-cell recordings from visually identified and biocytin-labeled subplate cells in somatosensory cortical slices from postnatal day 0–3 rats. Subplate neurons had an average resting membrane potential of –55 mV and input resistance of ~1.1 G{Omega}. Suprathreshold current injection elicited in 67% of the cells repetitive action potentials at 4–13 Hz and the remaining 33% showed only one spike. Three classes of spontaneous postsynaptic currents (sPSCs) could be identified: (i) Fast sPSCs, with an average amplitude of 14 pA and a decay time of 6.3 ms, which showed a 95% decrease in their frequency during (±)-{gamma}-amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid (AMPA)/kainate receptor blockade. Cyclothiazide caused a 3.5-fold increase in the decay time, indicating that fast sPSCs were mediated by AMPA receptors. (ii) Slow sPSCs, with 18 pA amplitude and 51.2 ms decay time were blocked by the N-methyl-D-aspartate (NMDA) receptor antagonist CPP. (iii) Chloride-driven sPSCs, with 34.4 pA amplitude and 123 ms decay time that were blocked by the {gamma}-amino-butyric acid A (GABAA) receptor antagonist gabazine. While tetrodotoxin citrate (TTX) blocked completely NMDA-mediated slow sPSCs, the frequency of AMPA- and GABAA-mediated sPSCs was reduced in TTX by 55 and 90%, respectively. These results indicate that subplate neurons receive functional synaptic inputs mediated by AMPA, NMDA and GABAA receptors.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Neurophysiol.Home page
W. Kilb, I. L. Hanganu, A. Okabe, B. A. Sava, C. Shimizu-Okabe, A. Fukuda, and H. J. Luhmann
Glycine Receptors Mediate Excitation of Subplate Neurons in Neonatal Rat Cerebral Cortex
J Neurophysiol, August 1, 2008; 100(2): 698 - 707.
[Abstract] [Full Text] [PDF]

Home page
 Cereb CortexHome page
N. Bayatti, J. A. Moss, L. Sun, P. Ambrose, J. F. H. Ward, S. Lindsay, and G. J. Clowry
A Molecular Neuroanatomical Study of the Developing Human Neocortex from 8 to 17 Postconceptional Weeks Revealing the Early Differentiation of the Subplate and Subventricular Zone
Cereb Cortex, July 1, 2008; 18(7): 1536 - 1548.
[Abstract] [Full Text] [PDF]

Home page
 Cereb CortexHome page
I. L. Hanganu, A. Okabe, V. Lessmann, and H. J. Luhmann
Cellular Mechanisms of Subplate-Driven and Cholinergic Input-Dependent Network Activity in the Neonatal Rat Somatosensory Cortex
Cereb Cortex, April 24, 2008; (2008) bhn061v1.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. Torres-Reveron and M. J. Friedlander
Properties of Persistent Postnatal Cortical Subplate Neurons
J. Neurosci., September 12, 2007; 27(37): 9962 - 9974.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
S. Higashi, K. Hioki, T. Kurotani, N. Kasim, and Z. Molnar
Functional Thalamocortical Synapse Reorganization from Subplate to Layer IV during Postnatal Development in the Reeler-Like Mutant Rat (Shaking Rat Kawasaki)
J. Neurosci., February 9, 2005; 25(6): 1395 - 1406.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
I. L. Hanganu and H. J. Luhmann
Functional Nicotinic Acetylcholine Receptors on Subplate Neurons in Neonatal Rat Somatosensory Cortex
J Neurophysiol, July 1, 2004; 92(1): 189 - 198.
[Abstract] [Full Text] [PDF]

Home page
 Cereb CortexHome page
M. Melone, F. Quagliano, P. Barbaresi, H. Varoqui, J. D. Erickson, and F. Conti
Localization of the Glutamine Transporter SNAT1 in Rat Cerebral Cortex and Neighboring Structures, With a Note on its Localization in Human Cortex
Cereb Cortex, May 1, 2004; 14(5): 562 - 574.
[Abstract] [Full Text] [PDF]

Home page
 Cereb CortexHome page
A. E. Csillik, E. Okuno, B. Csillik, E. Knyihar, and L. Vecsei
Expression of Kynurenine Aminotransferase in the Subplate of the Rat and Its Possible Role in the Regulation of Programmed Cell Death
Cereb Cortex, November 1, 2002; 12(11): 1193 - 1201.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
I. L. Hanganu, W. Kilb, and H. J. Luhmann
Functional Synaptic Projections onto Subplate Neurons in Neonatal Rat Somatosensory Cortex
J. Neurosci., August 15, 2002; 22(16): 7165 - 7176.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.