Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (18)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Maric, D.
Right arrow Articles by Barker, J. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Maric, D.
Right arrow Articles by Barker, J. L.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Cerebral Cortex, Vol. 10, No. 8, 729-747, August 2000
© 2000 Oxford University Press

Stereotypical Physiological Properties Emerge During Early Neuronal and Glial Lineage Development in the Embryonic Rat Neocortex

Dragan Maric, Irina Maric, Yoong Hee Chang and Jeffery L. Barker

Laboratory of Neurophysiology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA

Surface immunolabeling was used together with membrane potential and/or Ca2+ indicator dyes to characterize physiological properties emerging among precursors, neuroglial progenitors and differentiating neurons during neurogenesis of embryonic rat neocortex. Cells were immunoidentified with tetanus toxin (TnTx), which binds to gangliosides expressed by neurons, and anti-A2B5, which reacts with gangliosides expressed by neuroglial progenitors. Microdissection of the neocortex into ventricular/subventricular zone (VZ/SVZ) and cortical plate/subplate (CP/SP) regions further resolved the TnTx/A2B5-immunoidentified cells into pre- and post-migratory subpopulations. Quantitative immunocytochemistry revealed mainly proliferative (BrdU+) and immature (nestin+) elements among TnTx–A2B5– precursors and TnTx–A2B5+ progenitors in the VZ/SVZ, and the appearance of neuron-specific antigens among post-mitotic TnTx+ subpopulations of the CP/SP. Flow cytometry of acutely prepared cells in suspension and dual-imaging of cells in culture revealed that ionotropic amino acid receptors and metabotropic acetylcholine receptors closely paralleled the emergence of voltage-dependent Na+ and Ca2+ channels and Na+–Ca2+ exchange activity among TnTx+ neuronal progenitors migrating from VZ/SVZ to CP/SP. During this period, TnTx–A2B5– precursors and TnTx–A2B5+ neuroglial progenitors from VZ/SVZ predominantly exhibited Ca2+ responses to ATP. Thus, stereotypical and contrasting physiologies emerge among embryonic cortical cells in vivo as they initially progress from proliferating precursors and progenitors along neuronal and glial cell lineages.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Physiol. Rev.Home page
G. Burnstock
Physiology and Pathophysiology of Purinergic Neurotransmission
Physiol Rev, April 1, 2007; 87(2): 659 - 797.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
D. Maric, A. Fiorio Pla, Y. H. Chang, and J. L. Barker
Self-Renewing and Differentiating Properties of Cortical Neural Stem Cells Are Selectively Regulated by Basic Fibroblast Growth Factor (FGF) Signaling via Specific FGF Receptors
J. Neurosci., February 21, 2007; 27(8): 1836 - 1852.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. S. Gal, Y. M. Morozov, A. E. Ayoub, M. Chatterjee, P. Rakic, and T. F. Haydar
Molecular and Morphological Heterogeneity of Neural Precursors in the Mouse Neocortical Proliferative Zones
J. Neurosci., January 18, 2006; 26(3): 1045 - 1056.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J.-C. Platel, S. Boisseau, A. Dupuis, J. Brocard, A. Poupard, M. Savasta, M. Villaz, and M. Albrieux
Na+ channel-mediated Ca2+ entry leads to glutamate secretion in mouse neocortical preplate
PNAS, December 27, 2005; 102(52): 19174 - 19179.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
W. J. Moody and M. M. Bosma
Ion Channel Development, Spontaneous Activity, and Activity-Dependent Development in Nerve and Muscle Cells
Physiol Rev, July 1, 2005; 85(3): 883 - 941.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
A. F. Pla, D. Maric, S.-C. Brazer, P. Giacobini, X. Liu, Y. H. Chang, I. S. Ambudkar, and J. L. Barker
Canonical Transient Receptor Potential 1 Plays a Role in Basic Fibroblast Growth Factor (bFGF)/FGF Receptor-1-Induced Ca2+ Entry and Embryonic Rat Neural Stem Cell Proliferation
J. Neurosci., March 9, 2005; 25(10): 2687 - 2701.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
D. Maric, I. Maric, Y. H. Chang, and J. L. Barker
Prospective Cell Sorting of Embryonic Rat Neural Stem Cells and Neuronal and Glial Progenitors Reveals Selective Effects of Basic Fibroblast Growth Factor and Epidermal Growth Factor on Self-Renewal and Differentiation
J. Neurosci., January 1, 2003; 23(1): 240 - 251.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
D. Maric, Q.-Y. Liu, I. Maric, S. Chaudry, Y.-H. Chang, S. V. Smith, W. Sieghart, J.-M. Fritschy, and J. L. Barker
GABA Expression Dominates Neuronal Lineage Progression in the Embryonic Rat Neocortex and Facilitates Neurite Outgrowth via GABAA Autoreceptor/Cl{-} Channels
J. Neurosci., April 1, 2001; 21(7): 2343 - 2360.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.